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revertra acer kit  (Toyobo)


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    Structured Review

    Toyobo revertra acer kit
    Revertra Acer Kit, supplied by Toyobo, used in various techniques. Bioz Stars score: 99/100, based on 9068 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/revertra+acer+kit/ReverTra+Ace/pm40935787-112-14-17
    Average 99 stars, based on 9068 article reviews
    revertra acer kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Synthesized:

    Article Title: UMP Kinase Regulates Chloroplast Development and Cold Response in Rice
    Article Snippet: Total RNA was extracted from these rice tissues using an RNeasy Plus Mini Kit (QIAGEN, German). .. First-strand cDNA was synthesized using a ReverTra AceR kit (Toyobo, Japan). .. Quantitative real-time PCR was performed on an Applied Biosystems 7500 using SYBR qPCR Mix kit (Toyobo, Japan).

    Article Title: Control of Grain Shape and Size in Rice by Two Functional Alleles of OsPUB3 in Varied Genetic Background
    Article Snippet: Total RNA was extracted from leaves of MY46 using RNeasy Plus Mini Kit (QIAGEN, Hilden, German). .. The 1 st strand cDNA was synthesized using ReverTra AceR Kit (Toyobo, Osaka, Japan). ..

    Article Title: Importance of the Interaction between Heading Date Genes Hd1 and Ghd7 for Controlling Yield Traits in Rice
    Article Snippet: Total RNA was extracted using RNeasy Plus Mini Kit (QIAGEN, Hilden, German). .. First-strand cDNA was synthesized using ReverTra AceR Kit (Toyobo, Osaka, Japan). .. Quantitative real-time PCR was performed on Applied Biosystems 7500 using SYBR qPCR Mix Kit (Toyobo, Osaka, Japan) according to the manufacturer’s instructions.

    Article Title: Dissection and fine-mapping of two QTL for grain size linked in a 460-kb region on chromosome 1 of rice
    Article Snippet: Total RNA was extracted using RNeasy Plus Mini Kit (QIAGEN, German). .. First-strand cDNA was synthesized using ReverTra AceR Kit (TOYOBO, Japan). .. Quantitative real-time PCR was performed on Applied Biosystems 7500 using SYBR qPCR Mix Kit (TOYOBO, Japan) according to the manufacturer’s instructions.

    Article Title: Control of Thousand-Grain Weight by OsMADS56 in Rice
    Article Snippet: Total RNA was extracted using RNeasy Plus Mini Kit (Qiagen, Hilden, Germany). .. The first-strand complementary DNA was synthesized using ReverTra AceR Kit (Toyobo, Osaka, Japan). qRT-PCR was performed on Applied Biosystems 7500 using SYBR qPCR Mix Kit (Toyobo, Osaka, Japan). ..

    Generated:

    Article Title: Identification of qTGW2, a Minor-Effect QTL Controlling Grain Weight in Rice
    Article Snippet: .. The 1st strand cDNA was generated via qRT-PCR using the ReverTra Acer Kit (TOYOBO, Osaka, Japan). qRT-PCR was performed using the SYBR qPCR Mix Kit (TOYOBO, Osaka, Japan) on an ABI 7500 Real-Time PCR System, and data analysis was carried out using the 2_DDCt method. ..

    Quantitative RT-PCR:

    Article Title: Identification of qTGW2, a Minor-Effect QTL Controlling Grain Weight in Rice
    Article Snippet: .. The 1st strand cDNA was generated via qRT-PCR using the ReverTra Acer Kit (TOYOBO, Osaka, Japan). qRT-PCR was performed using the SYBR qPCR Mix Kit (TOYOBO, Osaka, Japan) on an ABI 7500 Real-Time PCR System, and data analysis was carried out using the 2_DDCt method. ..

    Article Title: Control of Thousand-Grain Weight by OsMADS56 in Rice
    Article Snippet: Total RNA was extracted using RNeasy Plus Mini Kit (Qiagen, Hilden, Germany). .. The first-strand complementary DNA was synthesized using ReverTra AceR Kit (Toyobo, Osaka, Japan). qRT-PCR was performed on Applied Biosystems 7500 using SYBR qPCR Mix Kit (Toyobo, Osaka, Japan). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Identification of qTGW2, a Minor-Effect QTL Controlling Grain Weight in Rice
    Article Snippet: .. The 1st strand cDNA was generated via qRT-PCR using the ReverTra Acer Kit (TOYOBO, Osaka, Japan). qRT-PCR was performed using the SYBR qPCR Mix Kit (TOYOBO, Osaka, Japan) on an ABI 7500 Real-Time PCR System, and data analysis was carried out using the 2_DDCt method. ..

    Article Title: Control of Thousand-Grain Weight by OsMADS56 in Rice
    Article Snippet: Total RNA was extracted using RNeasy Plus Mini Kit (Qiagen, Hilden, Germany). .. The first-strand complementary DNA was synthesized using ReverTra AceR Kit (Toyobo, Osaka, Japan). qRT-PCR was performed on Applied Biosystems 7500 using SYBR qPCR Mix Kit (Toyobo, Osaka, Japan). ..

    Isolation:

    Article Title: Unraveling Optimal Dose and Responsive Markers for Human Cord Blood Derived Mononuclear Cells in Alleviating Bronchopulmonary Dysplasia in Neonatal Mice.
    Article Snippet: .. Total RNA was isolated using Trizol method and reverse transcribed to cDNA using the ReverTra AceR kit (Toyobo, TRT-101). .. Quantitative real-time PCR reactions were conducted using SYBR Green PCR master mix (Toyobo, QPX-201) through ABI QSFlex6 system.

    Reverse Transcription:

    Article Title: Unraveling Optimal Dose and Responsive Markers for Human Cord Blood Derived Mononuclear Cells in Alleviating Bronchopulmonary Dysplasia in Neonatal Mice.
    Article Snippet: .. Total RNA was isolated using Trizol method and reverse transcribed to cDNA using the ReverTra AceR kit (Toyobo, TRT-101). .. Quantitative real-time PCR reactions were conducted using SYBR Green PCR master mix (Toyobo, QPX-201) through ABI QSFlex6 system.

    Article Title: Dissection of Two QTL for Grain Size Linked on the Long Arm of Chromosome 5 in Rice
    Article Snippet: RNA extraction and real-time PCR analysis Total RNA was isolated from 8 cm-long young panicles of NIL-TQ and NIL-IRBB52 using RNeasy Pius Mini Kit (QIAGEN, German). .. First-stand cDNA obtained via reverse transcription of total RNA using ReverTra AceR Kit (TOYOBO, Japan). .. Realtime PCR was conducted on Applied Biosystems 7500 using SYBR qPCR Mix Kit (TOYOBO, Japan) following the manufacturer’s instructions. qRT-PCR reaction was carried out with a total volume of 20 μL, consisting 2 μL cDNA, 10 μL SYBR qPCR Mix, 0.4 μL of each gene-speci c primer (Additional le 1: Table S3) and ddH2O.



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    revertra acer quantitative polymerase chain reaction qpcr reverse transcriptase rt  (Toyobo)
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    Enhanced at puberty 1 (EAP1) is an androgen receptor (AR) coactivator. A, LNCaP cells were transiently transfected with the pGL4.1-PSA-Luc reporter (500 ng) and pcDNA3.1-hemagglutinin (HA)-EAP1 expression vectors at the indicated amounts (ng). After 24 hours of incubation, the cells were stimulated with vehicle (ethanol) or 10-nM dihydrotestosterone (DHT) for 24 hours. Data were analyzed by one-way analysis of variance (ANOVA) with a post hoc Tukey-Kramer test (n = 4) Error bars indicate SDs. * P less than .05; ** P less than .01; *** P less than .001. HA-tagged lysine-specific demethylase 1 (LSD1), as an AR coactivator, was used as the positive control. B, LNCaP cells were transiently transfected with the pGL4.1-PSA-Luc reporter vector (500 ng) and pcDNA3.1 expressing either HA-tagged wild-type EAP1 or HA-tagged EAP1 C715A mutant at the indicated amounts. After 24 hours’ incubation, cells were stimulated with vehicle (ethanol) or 10-nM DHT for 24 hours. Data were analyzed by one-way ANOVA with a post hoc Tukey-Kramer test (n = 4) Error bars indicate SDs. ** P less than .01; n.s., not significant. C, Knockdown of EAP1 by short hairpin RNA (shRNA). Quantitative <t>polymerase</t> chain reaction <t>(qPCR)</t> analysis for EAP1 was performed. LNCaP cells expressing shLacZ were used as a negative control. Gene expression was normalized to that of Rplp0 and presented as the fold change in expression relative to the expression level in cells not treated with DHT. Data were analyzed by one-way ANOVA with a post hoc Tukey-Kramer test (n = 4) Error bars indicate SDs. * P less than .05; ** P less than .01. E, Knockdown of EAP1 by shRNA and its effect on the transcriptional activity of AR. Cells were treated with 10-nM DHT for 12 hours, and qPCR analysis of AR target genes was performed. Gene expression was normalized to that of Rplp0 and presented as the fold change in expression relative to the expression level in cells not treated with DHT. Data were analyzed by one-way ANOVA with a post hoc Tukey-Kramer test (n = 4) Error bars indicate SDs. * P less than .05; ** P less than .01; *** P less than .001; n.s., not significant. F, Chromatin immunoprecipitation analysis of EAP1 and AR at the indicated gene enhancers. DNA fragments in LNCaP cells were precipitated with anti-EAP1 and anti-AR; rabbit immunoglobulin G (IgG) was used as a negative control for the immunoprecipitation. Precipitated DNA fragments were assessed by qPCR. The error bars indicate SDs. Data were analyzed by one-way ANOVA with a post hoc Tukey-Kramer test (n = 3) Error bars indicate SDs. * P less than .05; *** P less than .001; n.s., not significant. G, Cell proliferation assays were performed using the WST-8 cell proliferation assay. The relative absorbance of each cell at the indicated time points is shown. Data were analyzed by one-way ANOVA with a post hoc Tukey-Kramer test (n = 5) Error bars indicate SDs. *** P less than .001.
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    Average 99 stars, based on 1 article reviews
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    Enhanced at puberty 1 (EAP1) is an androgen receptor (AR) coactivator. A, LNCaP cells were transiently transfected with the pGL4.1-PSA-Luc reporter (500 ng) and pcDNA3.1-hemagglutinin (HA)-EAP1 expression vectors at the indicated amounts (ng). After 24 hours of incubation, the cells were stimulated with vehicle (ethanol) or 10-nM dihydrotestosterone (DHT) for 24 hours. Data were analyzed by one-way analysis of variance (ANOVA) with a post hoc Tukey-Kramer test (n = 4) Error bars indicate SDs. * P less than .05; ** P less than .01; *** P less than .001. HA-tagged lysine-specific demethylase 1 (LSD1), as an AR coactivator, was used as the positive control. B, LNCaP cells were transiently transfected with the pGL4.1-PSA-Luc reporter vector (500 ng) and pcDNA3.1 expressing either HA-tagged wild-type EAP1 or HA-tagged EAP1 C715A mutant at the indicated amounts. After 24 hours’ incubation, cells were stimulated with vehicle (ethanol) or 10-nM DHT for 24 hours. Data were analyzed by one-way ANOVA with a post hoc Tukey-Kramer test (n = 4) Error bars indicate SDs. ** P less than .01; n.s., not significant. C, Knockdown of EAP1 by short hairpin RNA (shRNA). Quantitative polymerase chain reaction (qPCR) analysis for EAP1 was performed. LNCaP cells expressing shLacZ were used as a negative control. Gene expression was normalized to that of Rplp0 and presented as the fold change in expression relative to the expression level in cells not treated with DHT. Data were analyzed by one-way ANOVA with a post hoc Tukey-Kramer test (n = 4) Error bars indicate SDs. * P less than .05; ** P less than .01. E, Knockdown of EAP1 by shRNA and its effect on the transcriptional activity of AR. Cells were treated with 10-nM DHT for 12 hours, and qPCR analysis of AR target genes was performed. Gene expression was normalized to that of Rplp0 and presented as the fold change in expression relative to the expression level in cells not treated with DHT. Data were analyzed by one-way ANOVA with a post hoc Tukey-Kramer test (n = 4) Error bars indicate SDs. * P less than .05; ** P less than .01; *** P less than .001; n.s., not significant. F, Chromatin immunoprecipitation analysis of EAP1 and AR at the indicated gene enhancers. DNA fragments in LNCaP cells were precipitated with anti-EAP1 and anti-AR; rabbit immunoglobulin G (IgG) was used as a negative control for the immunoprecipitation. Precipitated DNA fragments were assessed by qPCR. The error bars indicate SDs. Data were analyzed by one-way ANOVA with a post hoc Tukey-Kramer test (n = 3) Error bars indicate SDs. * P less than .05; *** P less than .001; n.s., not significant. G, Cell proliferation assays were performed using the WST-8 cell proliferation assay. The relative absorbance of each cell at the indicated time points is shown. Data were analyzed by one-way ANOVA with a post hoc Tukey-Kramer test (n = 5) Error bars indicate SDs. *** P less than .001.

    Journal: Journal of the Endocrine Society

    Article Title: Identification and Functional Characterization of a Novel Androgen Receptor Coregulator, EAP1

    doi: 10.1210/jendso/bvab150

    Figure Lengend Snippet: Enhanced at puberty 1 (EAP1) is an androgen receptor (AR) coactivator. A, LNCaP cells were transiently transfected with the pGL4.1-PSA-Luc reporter (500 ng) and pcDNA3.1-hemagglutinin (HA)-EAP1 expression vectors at the indicated amounts (ng). After 24 hours of incubation, the cells were stimulated with vehicle (ethanol) or 10-nM dihydrotestosterone (DHT) for 24 hours. Data were analyzed by one-way analysis of variance (ANOVA) with a post hoc Tukey-Kramer test (n = 4) Error bars indicate SDs. * P less than .05; ** P less than .01; *** P less than .001. HA-tagged lysine-specific demethylase 1 (LSD1), as an AR coactivator, was used as the positive control. B, LNCaP cells were transiently transfected with the pGL4.1-PSA-Luc reporter vector (500 ng) and pcDNA3.1 expressing either HA-tagged wild-type EAP1 or HA-tagged EAP1 C715A mutant at the indicated amounts. After 24 hours’ incubation, cells were stimulated with vehicle (ethanol) or 10-nM DHT for 24 hours. Data were analyzed by one-way ANOVA with a post hoc Tukey-Kramer test (n = 4) Error bars indicate SDs. ** P less than .01; n.s., not significant. C, Knockdown of EAP1 by short hairpin RNA (shRNA). Quantitative polymerase chain reaction (qPCR) analysis for EAP1 was performed. LNCaP cells expressing shLacZ were used as a negative control. Gene expression was normalized to that of Rplp0 and presented as the fold change in expression relative to the expression level in cells not treated with DHT. Data were analyzed by one-way ANOVA with a post hoc Tukey-Kramer test (n = 4) Error bars indicate SDs. * P less than .05; ** P less than .01. E, Knockdown of EAP1 by shRNA and its effect on the transcriptional activity of AR. Cells were treated with 10-nM DHT for 12 hours, and qPCR analysis of AR target genes was performed. Gene expression was normalized to that of Rplp0 and presented as the fold change in expression relative to the expression level in cells not treated with DHT. Data were analyzed by one-way ANOVA with a post hoc Tukey-Kramer test (n = 4) Error bars indicate SDs. * P less than .05; ** P less than .01; *** P less than .001; n.s., not significant. F, Chromatin immunoprecipitation analysis of EAP1 and AR at the indicated gene enhancers. DNA fragments in LNCaP cells were precipitated with anti-EAP1 and anti-AR; rabbit immunoglobulin G (IgG) was used as a negative control for the immunoprecipitation. Precipitated DNA fragments were assessed by qPCR. The error bars indicate SDs. Data were analyzed by one-way ANOVA with a post hoc Tukey-Kramer test (n = 3) Error bars indicate SDs. * P less than .05; *** P less than .001; n.s., not significant. G, Cell proliferation assays were performed using the WST-8 cell proliferation assay. The relative absorbance of each cell at the indicated time points is shown. Data were analyzed by one-way ANOVA with a post hoc Tukey-Kramer test (n = 5) Error bars indicate SDs. *** P less than .001.

    Article Snippet: Total RNA was extracted by Sepasol RNA I Super G (Nacalai Tesque) and cDNA synthesized using ReverTra AceR quantitative polymerase chain reaction (qPCR) reverse transcriptase (RT) Master Mix (TOYOBO). qPCR was performed with the Thermal Cycler Dice Real Time System II (Takara Bio) according to the manufacturer’s instructions.

    Techniques: Transfection, Expressing, Incubation, Positive Control, Plasmid Preparation, Mutagenesis, Knockdown, shRNA, Real-time Polymerase Chain Reaction, Negative Control, Gene Expression, Activity Assay, Chromatin Immunoprecipitation, Immunoprecipitation, Proliferation Assay